Publications
- Ligasová, A., Vydržalová, M., Buriánová, R., Brůčková, L., Večeřová, R., Janošťáková, A., Koberna, K. A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 2019, 8, 1510
- Ligasová, A., Koberna, K., Quantification of fixed adherent cells using a strong enhancer of the fluorescence of DNA dyes (2019) Scientific Reports 9:8701
- Ligasová, A., Koberna, K., DNA Replication: From Radioisotopes to Click Chemistry (2018) Molecules 23(11). pii: E3007
- Ligasová et al., Cell cycle profiling by image and flow cytometry: The optimised protocol for the detection of replicational activity using 5-Bromo-2′-deoxyuridine, low concentration of hydrochloric acid and exonuclease III (2017) PLoS One 12(4): e0175880
- Ligasová et al., Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication (2017) PLoS One 12(3): e0174893
- Ligasová et al., Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2′-deoxyuridine and 5-ethynyl-2′-deoxycytidine (2016) Open Biology 6(1): 1-11
- Ligasová, A et al. The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2′-Deoxyuridine in Cellular DNA under Various Conditions (2015) PLoS One, (10)7: e0132393
- Ligasová, A., et al. A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity (2015) PLoS One, (10)2: e0117459
- Ligasová, A., et al. A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA (2013) PLoS One, 8(6):e66864
- Ligasová, A., et al. Atomic scissors: a new method of tracking the 5-bromo-2′-deoxyuridine-labeled DNA in situ (2012) PLoS One, 7(12):e52584
- Liboska, R., et al. Most anti-BrdU antibodies react with 2′-deoxy-5-ethynyluridine — the method for the effective suppression of this cross-reactivity (2012) PLoS One, 7(12):e51679
- Ligasová, A., et al. Secretory glands in cercaria of the neuropathogenic schistosome Trichobilharzia regenti – ultrastructural characterization, 3-D modelling, volume and pH estimations (2011) Parasit. Vectors, 4:162
- Ligasová, A., Koberna K. In situ reverse transcription: the magic of strength and anonymity (2010) Nucleic Acids Res., 38(16):e167
- Ligasová, A., et al. Organization of human replicon: singles or zipping couples? (2009) J. Struct. Biol., 165(3):204-213
- Cvačková, Z., et al. Pontin is localized in nucleolar fibrillar centers (2008) Chromosoma, 117(5):487-497
- Kalmárová, M., et al. Positioning of NORs and NOR-bearing chromosomes in relation to nucleoli (2007) J. Struct. Biol., 160(1):49-56
- Stavreva, D. A., et al. Potential roles for ubiquitin and the proteasome during ribosome biogenesis (2006) Mol. Cell Biol., 26:5131-5145
- Smirnov, E., et al. NORs and their transcription competence during the cell cycle (2006) Folia Biol., 52:59-70
- Fidlerová ,H., et al. Replication-coupled modulation of early replicating chromatin domains detected by anti-actin antibody (2005) J. Cell Biochem., 94:899-916
- Pliss, A., et al. Spatio-temporal dynamics at rDNA foci: global switching between DNA replication and transcription (2005) J. Cell Biochem., 15:554-565
- Li, J., et al. The chromatin remodeling complex NoRC controls replication timing of rRNA genes (2005) EMBO J., 12:120-127
- Koberna, K., et al. Electron microscopy of DNA replication in 3-D: evidence for similar-sized replication foci throughout S-phase (2005) J. Cell Biochem., 94(1):126-138
- Večeřová, J., et al. Formation of nuclear splicing factor compartments is independent of lamins A/C (2004) Mol. Biol. Cell, 15:4904-4910
- Raška, I., et al. The nucleolus and transcription of ribosomal genes (2004) Biol. Cell, 96:579-594
- Malínský, J., et al. Searching for active ribosomal genes in situ: light microscopy in light of the electron beam (2002) J. Struct. Biol., 140:227-231
- Koberna, K., et al. Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of “Christmas trees” in situ (2002) J. Cell Biol., 157:743-748
- Kopský, V., et al. An ATP-dependent step is required for the translocation of microinjected precursor mRNA into nuclear speckles (2002) Fol. Biol. (Prague), 48:69-72
- Staněk, D., et al. Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus (2001) Chromosoma, 110(7):460-470
- Malínský, J., et al. The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase (2001) J. Cell Sci., 114(Pt 4):747-750
- Koberna, K., et al., In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA (2000) Acta Histochem., 102(1):15-20
- Melčák, I., et al. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs (2000) Mol. Biol. Cell., 11(2):497-510
- Koberna, K., et al. Nuclear organization studied with the help of a hypotonic shift: its use permits hydrophilic molecules to enter into living cells (1999) Chromosoma, 108(5):325-335
- Koberna, K., et al. Non-isotopic detection of nucleolar transcription in pre-implantation mouse embryos (1998) Reprod. Nutr. Dev., 38(1):117-126
- Schul, W., et al. The RNA 3′ cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA (1996) EMBO J., 15(11):2883-2892
- Raška, I., et al. Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal (1995) J. Struct. Biol., 114(1):1-22
- Raška, I., et al. Structure-function subcompartments of the mammalian cell nucleus as revealed by the electron microscopic affinity cytochemistry (1992) Cell Biol. Int. Rep., 16(8):771-789
- Raška, I., et al. Ultrastructural immunolocalization of cyclin/PCNA in synchronized 3T3 cells (1989) Exp. Cell Res., 184(1):81-89
