Publications
Ligasová, A., Vydržalová, M., Buriánová, R., Brůčková, L., Večeřová, R., Janošťáková, A., Koberna, K. A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 2019, 8, 1510
Ligasová, A., Koberna, K., Quantification of fixed adherent cells using a strong enhancer of the fluorescence of DNA dyes (2019) Scientific Reports 9:8701
Ligasová, A., Koberna, K., DNA Replication: From Radioisotopes to Click Chemistry (2018) Molecules 23(11). pii: E3007
Ligasová et al., Cell cycle profiling by image and flow cytometry: The optimised protocol for the detection of replicational activity using 5-Bromo-2′-deoxyuridine, low concentration of hydrochloric acid and exonuclease III (2017) PLoS One 12(4): e0175880
Ligasová et al., Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication (2017) PLoS One 12(3): e0174893
Ligasová et al., Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2′-deoxyuridine and 5-ethynyl-2′-deoxycytidine (2016) Open Biology 6(1): 1-11
Ligasová, A et al. The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2′-Deoxyuridine in Cellular DNA under Various Conditions (2015) PLoS One, (10)7: e0132393
Ligasová, A., et al. A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity (2015) PLoS One, (10)2: e0117459
Ligasová, A., et al. A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA (2013) PLoS One, 8(6):e66864
Ligasová, A., et al. Atomic scissors: a new method of tracking the 5-bromo-2′-deoxyuridine-labeled DNA in situ (2012) PLoS One, 7(12):e52584
Liboska, R., et al. Most anti-BrdU antibodies react with 2′-deoxy-5-ethynyluridine — the method for the effective suppression of this cross-reactivity (2012) PLoS One, 7(12):e51679
Ligasová, A., et al. Secretory glands in cercaria of the neuropathogenic schistosome Trichobilharzia regenti – ultrastructural characterization, 3-D modelling, volume and pH estimations (2011) Parasit. Vectors, 4:162
Ligasová, A., Koberna K. In situ reverse transcription: the magic of strength and anonymity (2010) Nucleic Acids Res., 38(16):e167
Ligasová, A., et al. Organization of human replicon: singles or zipping couples? (2009) J. Struct. Biol., 165(3):204-213
Cvačková, Z., et al. Pontin is localized in nucleolar fibrillar centers (2008) Chromosoma, 117(5):487-497
Kalmárová, M., et al. Positioning of NORs and NOR-bearing chromosomes in relation to nucleoli (2007) J. Struct. Biol., 160(1):49-56
Stavreva, D. A., et al. Potential roles for ubiquitin and the proteasome during ribosome biogenesis (2006) Mol. Cell Biol., 26:5131-5145
Smirnov, E., et al. NORs and their transcription competence during the cell cycle (2006) Folia Biol., 52:59-70
Fidlerová ,H., et al. Replication-coupled modulation of early replicating chromatin domains detected by anti-actin antibody (2005) J. Cell Biochem., 94:899-916
Pliss, A., et al. Spatio-temporal dynamics at rDNA foci: global switching between DNA replication and transcription (2005) J. Cell Biochem., 15:554-565
Li, J., et al. The chromatin remodeling complex NoRC controls replication timing of rRNA genes (2005) EMBO J., 12:120-127
Koberna, K., et al. Electron microscopy of DNA replication in 3-D: evidence for similar-sized replication foci throughout S-phase (2005) J. Cell Biochem., 94(1):126-138
Večeřová, J., et al. Formation of nuclear splicing factor compartments is independent of lamins A/C (2004) Mol. Biol. Cell, 15:4904-4910
Raška, I., et al. The nucleolus and transcription of ribosomal genes (2004) Biol. Cell, 96:579-594
Malínský, J., et al. Searching for active ribosomal genes in situ: light microscopy in light of the electron beam (2002) J. Struct. Biol., 140:227-231
Koberna, K., et al. Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of “Christmas trees” in situ (2002) J. Cell Biol., 157:743-748
Kopský, V., et al. An ATP-dependent step is required for the translocation of microinjected precursor mRNA into nuclear speckles (2002) Fol. Biol. (Prague), 48:69-72
Staněk, D., et al. Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus (2001) Chromosoma, 110(7):460-470
Malínský, J., et al. The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase (2001) J. Cell Sci., 114(Pt 4):747-750
Koberna, K., et al., In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA (2000) Acta Histochem., 102(1):15-20
Melčák, I., et al. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs (2000) Mol. Biol. Cell., 11(2):497-510
Koberna, K., et al. Nuclear organization studied with the help of a hypotonic shift: its use permits hydrophilic molecules to enter into living cells (1999) Chromosoma, 108(5):325-335
Koberna, K., et al. Non-isotopic detection of nucleolar transcription in pre-implantation mouse embryos (1998) Reprod. Nutr. Dev., 38(1):117-126
Schul, W., et al. The RNA 3′ cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA (1996) EMBO J., 15(11):2883-2892
Raška, I., et al. Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal (1995) J. Struct. Biol., 114(1):1-22
Raška, I., et al. Structure-function subcompartments of the mammalian cell nucleus as revealed by the electron microscopic affinity cytochemistry (1992) Cell Biol. Int. Rep., 16(8):771-789
Raška, I., et al. Ultrastructural immunolocalization of cyclin/PCNA in synchronized 3T3 cells (1989) Exp. Cell Res., 184(1):81-89