{"id":341,"date":"2017-05-03T10:01:15","date_gmt":"2017-05-03T10:01:15","guid":{"rendered":"http:\/\/lucky-lab.eu\/?p=341"},"modified":"2017-08-07T14:18:57","modified_gmt":"2017-08-07T14:18:57","slug":"341","status":"publish","type":"post","link":"http:\/\/lucky-lab.eu\/?p=341","title":{"rendered":""},"content":{"rendered":"<p><a href=\"http:\/\/lucky-lab.eu\/?page_id=105\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"151\" data-permalink=\"http:\/\/lucky-lab.eu\/?attachment_id=151\" data-orig-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?fit=1772%2C989\" data-orig-size=\"1772,989\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"modularni_03 kopie\" data-image-description=\"\" data-image-caption=\"\" data-medium-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?fit=300%2C167\" data-large-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?fit=800%2C447\" class=\"alignnone size-medium wp-image-151\" src=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?resize=300%2C167\" alt=\"\" width=\"300\" height=\"167\" srcset=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?resize=300%2C167 300w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?resize=768%2C429 768w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?resize=1024%2C572 1024w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?resize=250%2C140 250w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?resize=150%2C84 150w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?w=1772 1772w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/modularni_03-kopie-1.png?w=1600 1600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><a href=\"http:\/\/lucky-lab.eu\/?page_id=108\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"158\" data-permalink=\"http:\/\/lucky-lab.eu\/?attachment_id=158\" data-orig-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?fit=806%2C945\" data-orig-size=\"806,945\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"front image\" data-image-description=\"\" data-image-caption=\"\" data-medium-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?fit=256%2C300\" data-large-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?fit=800%2C938\" class=\"alignnone wp-image-158\" src=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?resize=151%2C177\" alt=\"\" width=\"151\" height=\"177\" srcset=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?resize=256%2C300 256w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?resize=768%2C900 768w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?resize=128%2C150 128w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/front-image-1.png?w=806 806w\" sizes=\"auto, (max-width: 151px) 100vw, 151px\" \/><\/a><a href=\"http:\/\/lucky-lab.eu\/?page_id=207\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"347\" data-permalink=\"http:\/\/lucky-lab.eu\/?attachment_id=347\" data-orig-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/05\/Bez-n%C3%A1zvu-1.png?fit=300%2C167\" data-orig-size=\"300,167\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"Bez n\u00e1zvu-1\" data-image-description=\"\" data-image-caption=\"\" data-medium-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/05\/Bez-n%C3%A1zvu-1.png?fit=300%2C167\" data-large-file=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/05\/Bez-n%C3%A1zvu-1.png?fit=300%2C167\" class=\"alignnone wp-image-347 size-medium\" src=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/05\/Bez-n%C3%A1zvu-1.png?resize=300%2C167\" alt=\"\" width=\"300\" height=\"167\" srcset=\"https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/05\/Bez-n%C3%A1zvu-1.png?w=300 300w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/05\/Bez-n%C3%A1zvu-1.png?resize=250%2C139 250w, https:\/\/i0.wp.com\/lucky-lab.eu\/wp-content\/uploads\/2017\/05\/Bez-n%C3%A1zvu-1.png?resize=150%2C84 150w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<ul>\n<li>\n<h3>These pages were created with the aim of sharing our experiences with some methods of cell biology especially in the field of DNA replication and cell cycle analyses. At this moment, you can find here the basic protocols for the detection of replicating cells with <a href=\"http:\/\/lucky-lab.eu\/?page_id=481\">5-bromo-2\u00b4-deoxyuridine (BrdU)<\/a> or <a href=\"http:\/\/lucky-lab.eu\/?page_id=597\">5-ethynyl-2\u00b4-deoxyuridine (EdU)<\/a> by flow cytometry or microscopy or <a href=\"http:\/\/lucky-lab.eu\/?page_id=623\">simultaneous detection of DNA replication with BrdU and EdU<\/a>. These protocols were optimized with respect to signal intensity and the effect on cell structure. We provided also the protocol for the detection of <a href=\"http:\/\/lucky-lab.eu\/?page_id=644\">mitochondrial DNA and their replication <em>in situ<\/em> using BrdU<\/a>, the protocol for the fast and sensitive detection of <a href=\"http:\/\/lucky-lab.eu\/?page_id=712\">polyadenylated RNA <em>in situ<\/em><\/a>, the protocol for introduction of small molecules into cells using <a href=\"http:\/\/lucky-lab.eu\/?page_id=499\">hypotonic shift<\/a>, <a href=\"http:\/\/lucky-lab.eu\/?page_id=534\">recipes for the preparation of basic solutions<\/a> used in the described protocols<strong>\u00a0<\/strong>and also some <a href=\"http:\/\/lucky-lab.eu\/?page_id=504\">tips\u00a0for the selection of the convenient protocol<\/a> for the detection of replicating cells.<\/h3>\n<\/li>\n<li>\n<h3>Simultaneously, we would like to present two devices simplifying the laboratory work with <a href=\"http:\/\/lucky-lab.eu\/?page_id=441\">magnetic separations<\/a> and <a href=\"http:\/\/lucky-lab.eu\/?page_id=436\">cytocentrifugation<\/a>. These devices were developed as we missed similar solutions on the market. In addition, they provided us with a cheaper way to process our samples.<\/h3>\n<\/li>\n<li>\n<h3>We believe that the described methods and devices can help both the beginners and also more experienced researchers in the field of cell biology. Simultaneously, we hope that we will be able to improve the content of the pages by further methods and tips in the future. We will be grateful for any ideas and comments as we know that the reaction of the readers is the best way to improve these pages. Please note that we are not native English speakers and therefore, we apologize for any discomfort when reading the text.<\/h3>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>These pages were created with the aim of sharing our experiences with some methods of cell biology especially in the field of DNA replication and cell cycle analyses. At this moment, you can find here the basic protocols for the detection of replicating cells with 5-bromo-2\u00b4-deoxyuridine (BrdU) or 5-ethynyl-2\u00b4-deoxyuridine (EdU) &hellip; <a class=\"continue-reading-link\" href=\"http:\/\/lucky-lab.eu\/?p=341\"> Continue reading <span class=\"meta-nav\">&rarr; <\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":true,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-341","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/s8vlwi-341","jetpack-related-posts":[],"_links":{"self":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/posts\/341","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=341"}],"version-history":[{"count":24,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/posts\/341\/revisions"}],"predecessor-version":[{"id":812,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/posts\/341\/revisions\/812"}],"wp:attachment":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=341"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=341"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=341"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}