{"id":209,"date":"2017-03-24T09:35:59","date_gmt":"2017-03-24T09:35:59","guid":{"rendered":"http:\/\/lucky-lab.eu\/?page_id=209"},"modified":"2024-07-01T15:27:05","modified_gmt":"2024-07-01T13:27:05","slug":"publications","status":"publish","type":"page","link":"http:\/\/lucky-lab.eu\/?page_id=209","title":{"rendered":"Publications"},"content":{"rendered":"<ul>\n<li><\/li>\n<li><a href=\"https:\/\/www.mdpi.com\/2073-4409\/8\/12\/1510\" target=\"_blank\" rel=\"noopener noreferrer\">Ligasov\u00e1, A., Vydr\u017ealov\u00e1, M., Buri\u00e1nov\u00e1, R., Br\u016f\u010dkov\u00e1, L., Ve\u010de\u0159ov\u00e1, R., Jano\u0161\u0165\u00e1kov\u00e1, A., Koberna, K. A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 2019, 8, 1510<\/a><\/li>\n<li><a href=\"https:\/\/www.nature.com\/articles\/s41598-019-45217-9.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Ligasov\u00e1, A., Koberna, K.,\u00a0Quantification of fixed adherent cells using a strong enhancer of the fluorescence of DNA dyes (2019) Scientific Reports\u00a09:8701<\/a><\/li>\n<li><a href=\"https:\/\/www.mdpi.com\/1420-3049\/23\/11\/3007\/htm\" target=\"_blank\" rel=\"noopener noreferrer\">Ligasov\u00e1, A., Koberna, K.,\u00a0DNA Replication: From Radioisotopes to Click Chemistry (2018) Molecules\u00a023(11). pii: E3007<\/a><\/li>\n<li><a href=\"http:\/\/journals.plos.org\/plosone\/article\/file?id=10.1371\/journal.pone.0175880&amp;type=printable\">Ligasov\u00e1 et al., Cell cycle profiling by image and flow cytometry: The optimised protocol for the detection of replicational activity using 5-Bromo-2\u2032-deoxyuridine, low concentration of hydrochloric acid and exonuclease III (2017) PLoS One 12(4): e0175880\u00a0<\/a><\/li>\n<li><a href=\"http:\/\/journals.plos.org\/plosone\/article\/file?id=10.1371\/journal.pone.0174893&amp;type=printable\">Ligasov\u00e1 et al., Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication (2017) PLoS One 12(3): e0174893<\/a><\/li>\n<li><a href=\"http:\/\/rsob.royalsocietypublishing.org\/content\/royopenbio\/6\/1\/150172.full.pdf\">Ligasov\u00e1 et al., Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2&#8242;-deoxyuridine and 5-ethynyl-2&#8242;-deoxycytidine (2016) Open Biology 6(1): 1-11<\/a><\/li>\n<li><a href=\"http:\/\/journals.plos.org\/plosone\/article\/file?id=10.1371\/journal.pone.0132393&amp;type=printable\">Ligasov\u00e1, A et al. The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2&#8242;-Deoxyuridine in Cellular DNA under Various Conditions (2015) PLoS One, (10)7: e0132393<\/a><\/li>\n<li><a href=\"http:\/\/journals.plos.org\/plosone\/article\/file?id=10.1371\/journal.pone.0117459&amp;type=printable\">Ligasov\u00e1, A., et al. A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity (2015) PLoS One, (10)2: e0117459<\/a><\/li>\n<li><a href=\"http:\/\/journals.plos.org\/plosone\/article\/file?id=10.1371\/journal.pone.0066864&amp;type=printable\">Ligasov\u00e1, A., et al. A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA\u00a0(2013) PLoS One, 8(6):e66864<\/a><\/li>\n<li><a href=\"http:\/\/journals.plos.org\/plosone\/article\/file?id=10.1371\/journal.pone.0052584&amp;type=printable\">Ligasov\u00e1, A., et al. Atomic scissors: a new method of tracking the 5-bromo-2&#8242;-deoxyuridine-labeled DNA in situ (2012) PLoS One, 7(12):e52584<\/a><\/li>\n<li><a href=\"http:\/\/journals.plos.org\/plosone\/article\/file?id=10.1371\/journal.pone.0051679&amp;type=printable\">Liboska, R., et al. Most anti-BrdU antibodies react with 2&#8242;-deoxy-5-ethynyluridine &#8212; the method for the effective suppression of this cross-reactivity (2012) PLoS One, 7(12):e51679<\/a><\/li>\n<li><a href=\"https:\/\/parasitesandvectors.biomedcentral.com\/track\/pdf\/10.1186\/1756-3305-4-162?site=parasitesandvectors.biomedcentral.com\" target=\"_blank\" rel=\"noopener noreferrer\">Ligasov\u00e1, A., et al. Secretory glands in cercaria of the neuropathogenic schistosome Trichobilharzia regenti &#8211; ultrastructural characterization, 3-D modelling, volume and pH estimations (2011) Parasit. Vectors, 4:162<\/a><\/li>\n<li><a href=\"https:\/\/watermark.silverchair.com\/gkq619.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAc4wggHKBgkqhkiG9w0BBwagggG7MIIBtwIBADCCAbAGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMa6JXvTQbW54Z6NwiAgEQgIIBgZkmZ86VPw0Tf_xy4XZkd8ipkWS6jOgcrWJTZkGfohPut9iaX0Vfgf7QAVqPez_TEucEYrNd267ltCNtv_4JjVApM0sL8cch3rsp8L9zgMj_E6OkRdRSOodS5G6zSXd8FFK30-n-upVT9sUlQXxugrlokzxkTz7Q2txaRjuPXiJTbk8LE_18pHJt_yoEijQoaFEIImk2PvHU6b9FkPgKhgdJhajNq4bW2hChXoFAI4oPoEFwb8Q9rUZlccDZmk7X3WM7Vitf4e9eNCi49A5Q04hADXx5-JtwXH6sG3qwsmtzoKZGyCCm39iLWPf4JYfWP8dzA2_qeHjek8difO-eXtH-nqws0Kg5AziKtAW--U4Tg-53shbtNM3nnvaoLfmYPBehCwdLKe9mS-MQZrsgJy5qwiu5WmaJXoXe1thZerHwQcsF30WZitbELL-Hyh-cj-QW8nIDpMdP_Zj-XQtFY3porpDI7kCg6mQzJNXFZPNA-CZ-eTLG4xbO0yqTS0_DUE8\">Ligasov\u00e1, A., Koberna K. In situ reverse transcription: the magic of strength and anonymity (2010) Nucleic Acids Res., 38(16):e167<\/a><\/li>\n<li><a href=\"http:\/\/ac.els-cdn.com\/S1047847708002682\/1-s2.0-S1047847708002682-main.pdf?_tid=c1d71972-1078-11e7-8c8a-00000aab0f6b&amp;acdnat=1490349829_34e971eac0d460fc5322d1017b192c89\">Ligasov\u00e1, A., et al. Organization of human replicon: singles or zipping couples? (2009) J. Struct. Biol., 165(3):204-213<\/a><\/li>\n<li><a href=\"http:\/\/download.springer.com\/static\/pdf\/713\/art%253A10.1007%252Fs00412-008-0170-8.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00412-008-0170-8&amp;token2=exp=1490350901~acl=%2Fstatic%2Fpdf%2F713%2Fart%25253A10.1007%25252Fs00412-008-0170-8.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs00412-008-0170-8*~hmac=7502ae304cb66b6d569eda2caef9bf81891948f20ab78f4f96d23d59e7b4f109\">Cva\u010dkov\u00e1, Z., et al. Pontin is localized in nucleolar fibrillar centers (2008) Chromosoma, 117(5):487-497<\/a><\/li>\n<li><a href=\"http:\/\/ac.els-cdn.com\/S1047847707001475\/1-s2.0-S1047847707001475-main.pdf?_tid=03ebb714-1079-11e7-b875-00000aacb361&amp;acdnat=1490349940_4a34e2549234ad9a9da3c23cbc80c471\">Kalm\u00e1rov\u00e1, M., et al. Positioning of NORs and NOR-bearing chromosomes in relation to nucleoli (2007) J. Struct. Biol., 160(1):49-56<\/a><\/li>\n<li><a href=\"http:\/\/mcb.asm.org\/content\/26\/13\/5131.full.pdf\">Stavreva, D. A., et al. Potential roles for ubiquitin and the proteasome during ribosome biogenesis (2006) Mol. Cell Biol., 26:5131-5145<\/a><\/li>\n<li><a href=\"http:\/\/fb.cuni.cz\/Data\/files\/folia_biologica\/volume_52_2006_3\/FB2006A0009.pdf\">Smirnov, E., et al. NORs and their transcription competence during the cell cycle (2006) Folia Biol., 52:59-70<\/a><\/li>\n<li><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.20374\/pdf\">Fidlerov\u00e1 ,H., et al. Replication-coupled modulation of early replicating chromatin domains detected by anti-actin antibody (2005) J. Cell Biochem., 94:899-916<\/a><\/li>\n<li><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.20317\/epdf\">Pliss, A., et al. Spatio-temporal dynamics at rDNA foci: global switching between DNA replication and transcription (2005) J. Cell Biochem., 15:554-565<\/a><\/li>\n<li><a href=\"http:\/\/emboj.embopress.org\/content\/embojnl\/24\/1\/120.full.pdf\">Li, J., et al. The chromatin remodeling complex NoRC controls replication timing of rRNA genes (2005) EMBO J., 12:120-127<\/a><\/li>\n<li><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.20300\/epdf\">Koberna, K., et al. Electron microscopy of DNA replication in 3-D: evidence for similar-sized replication foci throughout S-phase (2005) J. Cell Biochem., 94(1):126-138<\/a><\/li>\n<li><a href=\"http:\/\/www.molbiolcell.org\/content\/15\/11\/4904.full.pdf\">Ve\u010de\u0159ov\u00e1, J., et al. Formation of nuclear splicing factor compartments is independent of lamins A\/C (2004) Mol. Biol. Cell, 15:4904-4910<\/a><\/li>\n<li><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.biolcel.2004.04.015\/epdf\">Ra\u0161ka, I., et al. The nucleolus and transcription of ribosomal genes (2004) Biol. Cell, 96:579-594<\/a><\/li>\n<li><a href=\"http:\/\/ac.els-cdn.com\/S1047847702005749\/1-s2.0-S1047847702005749-main.pdf?_tid=be15dd5a-107d-11e7-bf2d-00000aab0f6b&amp;acdnat=1490351970_6bb7197916bc16de315bebd054ade046\">Mal\u00ednsk\u00fd, J., et al. Searching for active ribosomal genes in situ: light microscopy in light of the electron beam (2002) J. Struct. Biol., 140:227-231<\/a><\/li>\n<li><a href=\"http:\/\/jcb.rupress.org\/content\/jcb\/157\/5\/743.full.pdf\">Koberna, K., et al. Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of &#8220;Christmas trees&#8221; in situ (2002) J. Cell Biol., 157:743-748<\/a><\/li>\n<li><a href=\"http:\/\/lucky-lab.eu\/wp-content\/uploads\/2017\/03\/Kopsky-et-al..pdf\">Kopsk\u00fd, V., et al. An ATP-dependent step is required for the translocation of microinjected precursor mRNA into nuclear speckles (2002) Fol. Biol. (Prague), 48:69-72<\/a><\/li>\n<li><a href=\"http:\/\/download.springer.com\/static\/pdf\/852\/art%253A10.1007%252Fs00412-001-0172-2.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00412-001-0172-2&amp;token2=exp=1490353338~acl=%2Fstatic%2Fpdf%2F852%2Fart%25253A10.1007%25252Fs00412-001-0172-2.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs00412-001-0172-2*~hmac=8dee2f8d5e6cfc5ea02af5122b7f87ff75ea30a99b7edf274d2cf338ee818735\">Stan\u011bk, D., et al. Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus (2001) Chromosoma, 110(7):460-470<\/a><\/li>\n<li><a href=\"http:\/\/jcs.biologists.org\/content\/joces\/114\/4\/747.full.pdf\">Mal\u00ednsk\u00fd, J., et al. The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase (2001) J. Cell Sci., 114(Pt 4):747-750<\/a><\/li>\n<li><a href=\"http:\/\/ac.els-cdn.com\/S0065128104700124\/1-s2.0-S0065128104700124-main.pdf?_tid=e68db8a6-107e-11e7-adf0-00000aacb360&amp;acdnat=1490352468_dee7df1c571e65156c9e5d2f03170d49\">Koberna, K., et al., In situ fluorescence visualization of bromouridine incorporated into newly transcribed nucleolar RNA (2000) Acta Histochem., 102(1):15-20<\/a><\/li>\n<li><a href=\"http:\/\/www.molbiolcell.org\/content\/11\/2\/497.full.pdf\">Mel\u010d\u00e1k, I., et al. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs (2000) Mol. Biol. Cell., 11(2):497-510<\/a><\/li>\n<li><a href=\"http:\/\/download.springer.com\/static\/pdf\/104\/art%253A10.1007%252Fs004120050384.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs004120050384&amp;token2=exp=1496940268~acl=%2Fstatic%2Fpdf%2F104%2Fart%25253A10.1007%25252Fs004120050384.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs004120050384*~hmac=97e7e095c81cf972c55d88708e6778a3b3364737bec87b2c25ed8857e9dbd386\" target=\"_blank\" rel=\"noopener noreferrer\">Koberna, K., et al. Nuclear organization studied with the help of a hypotonic shift: its use permits hydrophilic molecules to enter into living cells (1999) Chromosoma, 108(5):325-335<\/a><\/li>\n<li><a href=\"http:\/\/rnd.edpsciences.org\/articles\/rnd\/pdf\/1998\/01\/RND_0926-5287_1998_38_1_ART0010.pdf\">Koberna, K., et al. Non-isotopic detection of nucleolar transcription in pre-implantation mouse embryos (1998) Reprod. Nutr. Dev., 38(1):117-126<\/a><\/li>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC450226\/pdf\/emboj00011-0273.pdf\">Schul, W., et al. The RNA 3&#8242; cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA (1996) EMBO J., 15(11):2883-2892<\/a><\/li>\n<li><a href=\"http:\/\/ac.els-cdn.com\/S1047847785710015\/1-s2.0-S1047847785710015-main.pdf?_tid=05031190-1080-11e7-85a8-00000aacb361&amp;acdnat=1490352948_a3171d8f5a74ff3c7114388c73d23e28\">Ra\u0161ka, I., et al. Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal (1995) J. Struct. Biol., 114(1):1-22<\/a><\/li>\n<li>Ra\u0161ka, I., et al. Structure-function subcompartments of the mammalian cell nucleus as revealed by the electron microscopic affinity cytochemistry (1992) Cell Biol. Int. Rep., 16(8):771-789<\/li>\n<li>Ra\u0161ka, I., et al. Ultrastructural immunolocalization of cyclin\/PCNA in synchronized 3T3 cells (1989) Exp. Cell Res., 184(1):81-89<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Ligasov\u00e1, A., Vydr\u017ealov\u00e1, M., Buri\u00e1nov\u00e1, R., Br\u016f\u010dkov\u00e1, L., Ve\u010de\u0159ov\u00e1, R., Jano\u0161\u0165\u00e1kov\u00e1, A., Koberna, K. A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 2019, 8, 1510 Ligasov\u00e1, A., Koberna, K.,\u00a0Quantification of fixed adherent cells using a strong enhancer of the fluorescence of DNA dyes (2019) Scientific &hellip; <a class=\"continue-reading-link\" href=\"http:\/\/lucky-lab.eu\/?page_id=209\"> Continue reading <span class=\"meta-nav\">&rarr; <\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":207,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-209","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P8vlwi-3n","jetpack-related-posts":[],"_links":{"self":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/pages\/209","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=209"}],"version-history":[{"count":44,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/pages\/209\/revisions"}],"predecessor-version":[{"id":882,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/pages\/209\/revisions\/882"}],"up":[{"embeddable":true,"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=\/wp\/v2\/pages\/207"}],"wp:attachment":[{"href":"http:\/\/lucky-lab.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=209"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}